IBTIKAR USIM | BIL. 4

I Bil 4 I Jun 2015 34 Novel Antifungal Peptides Produced by Leuconostoc mesenteroides DU15 Effectively Inhibit Growth of Aspergillus Title Zaiton Binti Hasan Author Article Document Type Journal of Food Science Journal Name 1.696 Impact Factor 2013 Q2 Quartile antifungal peptides; Aspergillus niger; lactic acid bacteria; Leuconostoc mesenteroides Keyword Purpose - The role of perceived organizational support (POS) and job satisfaction on Abstract The ability of Leuconostoc mesenteroides DU15 to produce antifungal peptides that inhibit growth of Aspergillus niger was evaluated under optimum growth conditions of 30 degrees C for 48 h. The cell- free supernatant showed inhibitory activity against A. niger. Five novel peptides were isolated with the sequences GPFPL, YVPLF, LLHGVPLP, GPFPLEMTLGPT, and TVYPFPGPL as identified by de novo sequencing using PEAKS 6 software. Peptide LLHGVPLP was the only positively charged (cationic peptides) and peptide GPFPLEMTLGPT negatively charged (anionic), whereas the rest are neutral. The identified peptides had high hydrophobicity ratio and low molecular weights with amino acids sequences ranging from 5 to 12 residues. The mode of action of these peptides is observed under the scanning electron microscope and is due to cell lysis of fungi. This work reveals the potential of peptides from L. mesenteroides DU15 as natural antifungal preservatives in inhibiting the growth of A. niger that is implicated to the spoilage during storage. PENERBITAN JURNAL